Screening and identification of rhizobacteria isolated

This article has been cited by other articles in PMC. The present study was conducted for isolation, screening, and identification of Azotobacter and Trichoderma from different soil samples. A total of 10 isolates of Azotobacter and Trichoderma were isolated from rhizospheric soils.

Screening and identification of rhizobacteria isolated

Screening and identification of rhizobacteria isolated

The present study was conducted for isolation, screening, and identification of Azotobacter and Trichoderma from different soil samples. A total of 10 isolates of Azotobacter and Trichoderma were isolated from rhizospheric soils.

The test isolates were biochemically characterized and screened in in-vitro conditions for their plant growth promoting properties. A total of 41 bands were scored, out of which 35 bands were found to be The influence of isolated plant growth promoting rhizobacteria PGPR strains on plant growth was studied using different parameters such as height of the plant, number of leaves, and number of branches, and bio-control activity was studied.

The present results concluded that the multiple beneficial activities of PGPR traits increase the plant growth and bio-control activity.

Azotobacter, bio-control activity, plant growth, plant growth promoting rhizobacteria, polymerase chain reaction-randomly amplified polymorphic DNA, trichoderma How to cite this article: Isolation, screening, and molecular characterization of plant growth promoting rhizobacteria isolates of Azotobacter and Trichoderma and their beneficial activities.

Screening and identification of rhizobacteria isolated

J Nat Sc Biol Med ;6: PGPR are diverse, complex, and important assemblages in the biosphere, [3] they are considered as a group of beneficial free-living soil bacteria for sustainable agriculture and environment.

Azotobacter is a free-living aerobic, nonsymbiotic nitrogen fixer and acts as PGPR helps in root expansion, improve uptake of plant nutrients, protects plants from root diseases and most important improves biomass production in the rhizosphere of almost all crops.

In view of this, the present study was undertaken to investigate the PGPR activity of strains Azotobacter and Trichoderma, isolated from different crop soils and used as biofertilizers for stimulation of plant growth and bio-control without damaging the environment.

Collection of soil samples A total of 10 isolates were isolated from rhizospheric soils.

Introduction

The pH of soil was determined in 1: Water ratio, keeping 30 min as equilibration time. Isolation and identification of isolates Collected soil samples were air-dried for 4 h, and isolation was done by serial dilution technique. Appearance of colonies was recorded, and individual colonies selected and maintained as a pure culture for further study.

The isolated colonies were sub-cultured and identified by using morphological and biochemical tests. The various tests were performed for Azotobacter like, catalase activity, motility test, oxidase activity, citrate test, indole test, methyl red test, Voges-Proskauer test, triple sugar ion test, nitrate reduction test.

Further it was extracted with equal volume of chloroform: Isoamyl alcohol and the aqueous phase was transferred to the fresh tube and to this equal volume of phenol: It was washed with chloroform: Isoamyl alcohol until the clear supernatant was obtained.

Screening of plant growth promoting Rhizobacteria isolated from - caninariojana.com

These reactions were repeated to check the reproducibility of the amplification. The banding pattern was visualized on ultraviolet transilluminator and documented by Alpha image analyzer. Effect of isolated strains on selected plants The isolated 10 samples were inoculated to Amaranthus spinosus and Stevia rebaudiana plants at 1:registration and adoption of PGPR for pest and disease management.

Key words: Carriers must be cheap and readily available for formulation development .. registration of biocontrol agents either by universities or private companies.

were observed which were isolated from different agroclimatic zones of Karnataka, [24] found % and [25] found 87% intra- specific genetic variation in Trichoderma isolates.

Screening and identification of rhizobacteria isolated from heavy metal contaminated soils INTRODUCTION As a result of human activities such as mining, agriculture and industrial activity, heavy metal pollution has become one of the most serious environmental problems today.

Introduction

were observed which were isolated from different agroclimatic zones of Karnataka, [24] found % and [25] found 87% intra- specific genetic variation in Trichoderma isolates. A. (). Isolation, characterization and identification of plant growth-promoting rhizobacteria.

International Journal of Agricultural In this study, a total of 25 nitrogen-fixing bacterial strains were isolated and screened for their growth-promoting activities such as indoleacetic acid (IAA) production, In vitro Screening of.

The influence of isolated plant growth promoting rhizobacteria (PGPR) strains on plant growth was studied using different parameters such as height of the plant, number of leaves, and number of branches, and bio-control activity was studied.